In Vitro Services

In vitro service platform

​

Whatever project is required for your studies, we have the in vitro service platform to support faster, better decision-making for your oncology, immuno-oncology, and inflammation disease drug discovery program.

 

• IC50 inhibition curves and proliferation studies to determine the half-maximal (50%) inhibitory concentration of cancer chemotherapeutics.

• In vitro drug screening. A robust suite of cell death, viability, proliferation, and other phenotypic assays to screen new compounds (small molecules and antibodies).

  • MTT assays

  • Cytotoxicity assays 

  • Clonogenic assays (2D/3D)

  • ELISA. Enzyme-linked immunosorbent assays to sensitively quantify key protein biomarkers.

•  SDS-Western Blot (protein immunoblot), is an antibody-based technique used to detect the presence, size and abundance of specific proteins (probed using target-specific antibodies) within a sample (target validation, target engagement)

• qPCR. Quantitative polymerase chain reaction technology to detect specific DNA and mRNA biomarkers.

 

In vitro/ex vivo pharmacology

NeoSome Life Sciences offers a full suite of custom in vitro and ex vivo pharmacology services to support faster screening and seamless translation from in vitro to in vivo studies.

• Patient-derived xenograft (PDX) and cell-line derived xenograft (CDX) models development

• Commercially available different cancer cell lines 

• Ex-vivo assays using PDX tumor tissue and other organs

• MSD (Meso Scale Discovery)

• Using with MSD (Meso Scale Discovery), electrochemiluminescence can measure normal and elevated levels of analytes within the same sample while delivering higher sensitivity than traditional ELISA. It tolerates an array of complex biological matrices including serum, plasma, tissue culture media, tumor tissue (target organ penetration) and accurately detects specific analyte levels in small sample volumes.

• NeoSome Life Sciences has MSD's experienced scientists to develop high-quality assays for your study applications and please talk to us about multiplex custom panel design and validation.

• Detect and Quantify Cytokines and Chemokines in High Throughput with our Multi-Analyte Profiling Platform

• NeoSome’s scientists most frequently utilize Meso Scale Discovery (MSD-ECL) in our work with biomarkers, immunogenicity, and cell-based assays.